Selective detection of N6-methyladenine in DNA via metal ion-mediated replication and rolling circle amplification† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6sc02271e Click here for additional data file.

Table S1 Primers and templates used in this strategy. Fig. S1 Effects of Ag + on the activity of KF exo-DNA polymerase. Fig. S2 The efficiencies of dCMP incorporation for A/6mA containing templates with different incubation time. Fig. S3 Primer extensions for A/6mA containing templates with different concentrations of Ag +. Fig. S4 The efficiencies of dCMP incorporation for A/6mA containing templates using increasing concentrations of KF exo-DNA polymerase. Fig. S5 Primer extensions for A/6mA containing templates using increasing concentrations of dCTP. Fig. S6 The efficiencies of dAMP incorporation for C, 5mC, 5hmC, 5fC and 5caC containing templates. Fig. S8 Single nucleotide incorporation using Taq DNA polymerase. Fig. S10 The effect of other metals ions compared with Ag + on the discrimination of 6mA from A for A/6mA-TA. Fig. S11 The effect of other metals ions compared with Ag + on the discrimination of 6mA from A for A/6mA-GG. Fig. S12 Discrimination of 6mA from A in double-stranded DNA templates using klenow exo-DNA polymerase without annealing step. Fig. S13 Discrimination of 6mA from A at 37°C using klenow exo-polymerase after annealing the dsDNA templates and primer.